Journal: Cells
Article Title: Molecular Genetics and Functional Analysis Implicate CDKN2BAS1-CDKN2B Involvement in POAG Pathogenesis
doi: 10.3390/cells9091934
Figure Lengend Snippet: CDKN2B-AS1 siRNA mediated knockdown in HEK293T and trabecular meshwork (TM) cells. ( a , b ) Relative quantification of CDKN2B-AS1 , CDKN2B , CDKN2A and P14ARF transcript in HEK293T and TM cells after CDKN2B-AS1 siRNA (10 nm) treatment when compared to control siRNA (10 nm) treatment for 72 h; ( c ) Representative cultures established after CDKN2B-AS1 and control siRNA treatment demonstrated increased CDKN2B (red) detection in phalloidin (green) stained HEK293T and TM cells. (Scale bar represents 30 µm; *** denotes p < 0.001; * denotes p < 0.01, Bar graphs illustrate fold change (2 −ΔΔ C T ) ± SEM).
Article Snippet: The HEK293T (human embryonic kidney-CRL3216) cells were obtained commercially from ATCC and primary human trabecular meshwork (hTM) cells (Lot # 3194) from Cell Applications.
Techniques: Knockdown, Quantitative Proteomics, Control, Staining